Control of Maximum Sarcoplasmic Reticulum Ca Load in Intact Ferret Ventricular Myocytes

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Control of Maximum Sarcoplasmic Reticulum Ca Load in Intact Ferret Ventricular Myocytes

In steady state, the Ca content of the sarcoplasmic reticulum (SR) of cardiac myocytes is determined by a balance among influx and efflux pathways. The SR Ca content may be limited mainly by the ATP-supplied chemical potential that is inherent in the gradient between SR and cytosol. That is, forward Ca pumping from cytosol to SR may be opposed by energetically conservative reverse pumping depen...

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Control of Maximum Sarcoplasmic Reticulum Ca Load in Intact Ferret Ventricular Myocytes Effects of Thapsigargin and Isoproterenol

In steady state, the Ca content of the sarcoplasmic reticulum (SR) of cardiac myocytes is determined by a balance among influx and efflux pathways. The SR Ca content may be limited mainly by the ATP-supplied chemical potential that is inherent in the gradient between SR and cytosol. That is, forward Ca pumping from cytosol to SR may be opposed by energetically conservative reverse pumping depen...

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Sarcoplasmic reticulum Ca release in intact ventricular myocytes.

Sarcoplasmic reticulum (SR) Ca release in intact ventricular myocytes is the major source of Ca which activates cardiac contraction (although Ca influx makes a non-negligible contribution in most species). The fundamental events of SR Ca release are known as Ca sparks. The twitch Ca transient is composed of approximately 10,000 Ca sparks occurring in a given cell, and they are synchronized by t...

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Sarcoplasmic Reticulum Ca Release by 4-Chloro-m-Cresol (4-CmC) in Intact and Chemically Skinned Ferret Cardiac Ventricular Fibers

The purpose of this study was to determine whether 4-chlorom-cresol (4-CmC) could generate caffeine-like responses in ferret cardiac muscle. The concentration dependence of 4-CmC-mediated release of Ca from the sarcoplasmic reticulum was studied in intact cardiac trabeculae and saponinskinned fibers in which the sarcoplasmic reticulum was loaded with Ca. In intact and saponin-skinned preparatio...

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Altered sarcoplasmic reticulum (SR) Ca2+-ATPase and Na+-Ca2+ exchange (NCX) function have been implicated in depressing SR Ca2+ content and contractile function in heart failure (HF). Enhanced diastolic ryanodine receptor (RyR) leak could also lower SR Ca2+ load in HF, but direct cellular measurements are lacking. In this study, we measure SR Ca2+ leak directly in intact isolated rabbit ventric...

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ژورنال

عنوان ژورنال: Journal of General Physiology

سال: 1998

ISSN: 0022-1295,1540-7748

DOI: 10.1085/jgp.111.4.491